Abstract: Presence of auxins and high humidity in the initial stages of growth in vitro of 10-15 days old shoot cuttings is very essential for adventitious rooting in microsperma lentil in cultivar Masoor-85. The type and quantity of auxins is important. The basal segments of stem have more chances of rooting which gradually decreases for apical segments. Also age of shoots in culture has influence on rooting. Rooting is with normal polarity. Boron ( 30 mg/l) and MS (1/10 strength) salts in diluted concentration helps root induction and development. Moist peat and filter paper bridge both support rooting.