Abstract: In vitro studies on physiology of four fungi namely Alternaria alternata, Colltotrichum gloeosporioides, Curvularia lunata and Fusarium pallidoroseum isolated from Cuscuta stem cuttings were conducted. All the fungi gave the best mycelial growth on universal medium. Out of these fungi A. alternata and F. pallidoroseum gave the best growth at 25°C and C. gloeosporioides gave the best mycelia! growth at 20°C while C. gloeosporioides required pH 6 for better mycelia! growth. 24 hours continuous light favoured the growth of A. alternata and C. glpeosporioides while C. lunata and F. pallidoroseum gave better results under 12 hours light and 12 hours darkness.