Abstract: Callogenesis and regenerative response of in-vivo leaf explant of tomato hybrids viz. `Bornia` and `Royesta` were studied at different Plant Growth Regulators (PGR)) levels. `Bornia` showed a high frequency of callogenesis than `Royesta` at various combinations of Benzyl Amino Purine (BAP) and Indol Acetic Acid (IAA). Maximum callogenesis (100%) was recorded on four different combinations of BAP and IAA. A media formulation with BAP @ 6.5 and IAA @ 11 μ M/l found to be optimum for both hybrids. `Royesta` regenerated higher percentage of shoots at BAP (6.5 μ M/l) alone and with IAA (6.0 μ M/l). Both hybrids regenerated roots at various concentrations of IAA alone.