Abstract: Availability of animal protein due to low production and high cost is limited. Therefore, vegetable protein being cheeper source, may be used in the preparation of weaning food for infants. The present study was carried out to prepare a weaning food consisting of sprouted wheat and protein isolate from an unconventional source, like stillage sludge. The chemical evaluation of weaning food was conducted. Amino acid profile of the protein isolate showed the chemical score was 36.42. However thyronine was the limiting amino acid. Results of biological trial conducted on weanling Albino rats showed the digestibility, 96.40, 97.83. Net protein utilization (NPU) 76.34, 78.8, biological value 79.20, 80.54 and protein efficiency ratio 4.0, 4.6 of skim milk diet respectively.