Abstract: The degree of bile tolerance and their action on conjugated bile salts were studied on six different strains of Bifidobacteria. They were Bifidobacterium infantis (ATCC 27920), B. adolescentis (ATCC 11146), B. breve (ATCC 15698), B. bihdurn (ATCC 35914) and B. longum (ATCC 15708). Sodium taurocholate was used as conjugated bile salt. The ability to grow in the presence of 0.3 percent bile salt varied among the six strains tested. B. longum (ATCC 15708) was the most tolerant strain.TPY broth containing 0.05 percent of sodium taurocholate was used to measure the deconjugation activity. All bifidobacteria tested were able to deconjugate conjugated bile salt but at various degrees. B. infantis (ATCC 27920) showed the highest deconjugation activity, where the cholic acid released was 0.015 μmol/ml.