Abstract: A Check list of 51 weeds, belonging to 27 families in Al-Kharj area of Saudi Arabia, was listed. All these weeds species were found in agricultural fields or in neglected farms. Most of these weeds cause moderate to severe infestations to various agricultural crops and fodder plants. Correct identification and the methods of eradication of these weeds from the agricultural fields are one of the primary objectives to improve the crop yields, further, a concise regional weed flora would serve much and help farmers in these regions. A brief description of each weed species is provided to make its identification easier.