Abstract: The present study was conducted to identify the soil mycoflora which could utilize the milk as a sole source of nitrogen and carbon. Soil samples collected from dairy farms were screened for fungi which could utilize milk powder as a sole source of carbon and nitrogen for their growth. Aspergillus was the predominant genus with 23 species followed by Penicillium with 21 species. Other dominant genera were Chaetomium (11 species), Eupenicillium (8 species), Cladosporium (5 species) and Chrysosporium (5 species). Altogether 131 fungal species belonging to 50 genera were isolated .