Abstract: Coat protein-mediated resistance (CP-MR) to potato mop-top furovirus (PMTV) was incorporated by transforming Nicotiana benthamiana, a susceptible host of PMTV, with sequences encoding the coat protein (CP) gene of the virus. The transgenic plants of N. benthamiana, expressing the PMTV gene were challenged with isolate T and isolate S of PMTV by mechanical, graft, and fungal inoculation. CP-MR against PMTV was extremely effective in coat protein gene transformed N. benthamiana plants. Transgenic T1 seedlings were inoculated in a bait test in which they were grown in soil containing viruliferous spores of the vector fungus Spongospora subterranea f. sp. subterranea. Virus transmission by its natural vector could only infect one or two plants of one transgenic line (W16) whereas four lines (W1, W2, W7, W25) were immune to fungally-mediated PMTV-infection. This type of resistance in potato crop will be very valuable because there is no other source of resistance or tolerance to PMTV.