Abstract: A large number of Bacillus thuringiensis (Bt) isolates separated from samples collected from different economic environments in Pakistan were characterized for Crystal Protein gene (cry) composition and pesticidal activity against devastating polyphagous pest, Helicoverpa armigera. Isolates harboring combinations cry2A genes were found me efficacious against the target pest. One isolate designated as PR17.4 (CEMB Bt) exhibited high levels of toxicity again H. armigera in lab biotoxicity assays and was therefore, chosen as a potential candidate in integrated Pest Management (1PM) strategies to control this notorious pest of valuable crops. Field efficacy of home grown cells CEMB Bt, PR17.4, Agr (50 WP), was compared with a commercial BT formulation of Novartis, growth regulators, Match (50 EC), Insegar (25 W and a popular chemical pesticide, Curacron (500 EC) to investigate their effectiveness to control H. armigera in potato crop Insegar was not significantly effective against the target pest. Locally isolated CEMB Bt was found to be as effective the commercial Bt and chemical control agent of Novartis. A synergistic effect was also observed among, Match/Agree CEMB Bt/Match, Match/Curacron and Agree/Curacron combinations. These studies suggest that CEMB Bt alone or combination with other biological or chemical based pesticides can be safely recommended for pest management strategy against H. armigera with no obvious harmful effects on its predators as is the case with chemical insecticides.