Abstract: This study was conducted to isolate and identify the EPS-producing bacteria associated with the roots of three wheat lines grown in saline and non-saline soils. Results indicated the presence of various EPS-producing bacterial genera in unplanted saline and non-saline soil, rhizosphere and rhizoplane of the three wheat fines. Bacillus sp. were more variable in unplanted saline than non-saline soil. In contrast to uniform distribution of Bacillus sp. in unplanted soils, other EPS-producing bacterial genera isolated from rhizosphere and rhizoplane were more numerous and diversified. Frequent occurrence of Microbacterium sp. in the soil and rhizosphere of the plants invoke the desire for more research work to explore the role of this bacterium in the saline environment.