Abstract: Vein thickening, enation formation and curling of leaves (upward or downward) are characteristic symptoms of cotton leaf curl virus, a whitefly (Bemisia tabaci) transmitted geminivirus, in cotton. To ascertain the effect of virus titer on type of curling and their relationship with epitope profile we determined virus concentration in 26 samples (13 each upward and downward curling) and established epitope profile of 34 samples (17 each) by TAS-ELISA using polyclonal antisera to Indian Cassava Mosaic Virus (ICMV) and Monoclonal Antibodies (MAbs) to African Cassava Mosaic Virus (ACMC) & ICMV and Okra Leaf Curl Virus (OLCV). No relationship between virus concentration and epitope profile was found with type of symptoms.