Abstract: Two strains KA-1 & AAN-2 of Saccharomyces cerevisiae were used as a tool for fermentation and the studies for optimization of different parameters for ethanol yield from corn steep liquor were undertaken. The maximum yield was obtained after 72 hr. at 30 C, 120 rpm in shake flask fermentation. Ethanol production by shake flask fermentation revealed that strain AAN-2 of S. cerevisiae had better results as compared to KA-1 which would be a good candidate of ethanol fermentation on industrial scale.