Abstract: Bifidobacterium isolates obtained from infants stools were assessed for their ability to survive in HCI solutions adjusted to pH levels of 1.0, 2.0, and 3.0. Five isolates (Bifidobacterium breve C20, B. infantis D22, B. infantis F91, B. infantis G4 and B. infantis Z45) out of 28 were able to survive at pH 1.0 after 0 h exposure. Survival was not detected after 1 h exposure to this pH level. At pH 2.0, only five isolates (B. infantis D22, B. infantis F66, B. infantis F34, B. breve F100 and B. infantis G4) survived after 1 h exposure. Four isolates (B. infantis D22, B. infantis F117, B. infantis F34 and B. infantis Z45) were able to survive at pH 3.0 during the 3 h incubation period. At pH 6.5 (control), essentially all isolates of bifidobacteria survived during 3 h incubation.