Abstract: Hypocotyl and callus of cotton (Gossypium hirsutum L. cv. Coker 201) were cultured on MS basal medium supplemented with various concentrations of NaCI in order to study the effects of salt stress on callus induction, growth, proliferation, embryogenesis and plant regeneration of cotton. Experiment results indicated that along with the increase of NaCI level, the percentage of induction, the growth rate, the survival rate, the moisture content, embryogenesis rate of cotton callus and the plant regeneration rate and normal seedling rate of cotton tissue culture decreased.