Abstract: Five cultivars viz; Purbi, Bedana, Bombai, Serai and Gola were collected to peer the chemical composition of Litchi (Litchi chinensis Sonn.) fruit as affected by fruit orientation on the tree. The fruits at Lower half were not only heavy in weight, pericarp and flesh but also had more percentage of total acidity. However, stone weight, pH value, vitamin C content and non-reducing sugars were found to be more in fruits collected from upper half portion of the tree. Hence it can be suggested that picking should be started from those fruits which are positioned at the lower half portion of the tree in order to collect fruits of superior quality.