Abstract: In vitro growth responses of various lentil genotypes varied. The best callussing response was noticed in variety ILL-4605. The genotype AARI-L-89503 had a tendency for embryogenesis. The genotype AARI-L-86642 had the best rooting ability. This genotype also showed in vitro flowering response. Masoor-85 and Pant-L-406 had moderate morphogenic responses, Meristem multiplication, shoot development, shoot bud formation from callus and adventitious rooting from shoot cuttings was obtained under appropriate growth regulators combinations.