Abstract:
Culture responses of 9 lentil explants were compared in various media having different ingredients. The most promising were the scrapped axillary portions of basal nodes for enhanced regeneration. The optimum size of the explant was also determined. The callogenesis response varied with the explant and ranged between 0.3-0.5 cm.
Among the sugars tested, sucrose was the best for callussing. Auxin 2,4-D induced callussing but cytokinin enhanced formationof shoot primordia in explants. Both BA and K promoted multiple shoot formation but K and GA gave more shoot buds. Cytokinins with auxins gave callusing. Callus was also induced in explants when grown in K (10 mg L–1) + GA (1 mg L–1) containing medium in the dark. When callus was shifted to light it started regeneration as green dots. The addition of seed extract to K and GA medium considerably enhanced regenerations.