Abstract: Rectal temperature, pulse rate and breath rate were recorded in mules at seven farms located at different places in Pakistan for consecutive 15 days. Rectal temperature (mean±standard error of the mean) was 99.7±0.15 in the morning, 100.1 ± 0.14 in the noon and 99.8 ± 0.19 in the evening. The pulse rate was 32.3 ± 1.14 in the morning, 34.1 ± 0.82 in the noon and 3.20 ± 1.02 in the evening. The breath rate was 11.7 ± 0.94 in the morning, 13.2 ± 0.91 in the noon and 11.6 ± 0.86 in the evening. There was a general tendency of higher rectal temperature, pulse rate and breath rate at noon than at morning or evening at all farms. However, the difference between farms and between varrious times of the day at any farm was statistically non-significant. Correlation between various parameters are studied. The study shows that mules do not seem to be influenced much by the geoclimate of the area.