Abstract: Background: The octapeptide angiotensin II (Ang II) is the major effector of the renin-angiotensin system. Ang II exerts its effects by binding to Ang II type 1 (AT1) and Ang II type 2 (AT2) receptors. To examine the involvement of Ang II and AT1 receptors in nociception the effects of Ang II, losartan (AT1 receptor antagonist) and combination (losartan+Ang II) infused uni-and bilaterally into hippocampal CA1 area of male Wistar rats were studied. Materials and Methods: After stereotaxic implantation of guide cannulae into hippocampal CA1 area Ang II (50 μg), losartan (100 μg) and combination (losartan 100 μg+Ang II 50 μg) were separately microinjected uni-and bilaterally into CA1 area. Nociception was examined applying mechanical pressure on the hind paw of the rat (paw pressure test). Results: It was found that bilateral and left-side microinjections of Ang II (50 μg) exerted a nociceptive effect. In contrast, the inhibition of AT1 receptors by losartan (100 μg), microinjected bilaterally and into the left CA1 area, produced an antinociceptive effect. The pretreatment with AT1 antagonist losartan infused bilaterally and into the left CA1 area reversed the nociceptive effect of Ang II as compared to the respective controls, i.e., elicited antinociception. The effect of all treatments was more pronounced after injection into the left CA1 area as compared to the right-side. Conclusion: The results suggest an involvement of hippocampal AT1 receptors in nociception of rats and a differential distribution of AT1 receptors in the left and right CA1 area.