Abstract: Background: Herbal preparations of Ficus amplissima had been considered as effective, economical and safe ethnomedicines for various ailments in Indian traditional system of medicine. The present study was aimed to explore scientifically the antioxidant, anti-inflammatory and wound healing potential of Ficus amplissima bark. Materials and Methods: In the present study, the methanol extract of F. amplissima bark were studied for enzymatic antioxidant activity through different assays, anti-inflammatory by using egg albumin induced inflammation and cotton pellet induced granuloma models (100 and 200 mg methanol extract) and wound healing activity by incorporating the two doses (1 and 2% (w/w)) of methanol extract and simple ointment base B.P. in concentration of 5% (w/w) using excision and incision wound models in rats. In case of the excision wound model, wound contraction and period of epithelization was studied while incision wound model was evaluated by determining tensile strength. Results: F. amplissima bark expressed the potent anti-inflammatory and in vivo antioxidant activity, where, 200 mg methanol extracts showed higher activity. Treatment of wound with ointment containing 2% (w/w) methanol extract was exhibited significant (p<0.001) wound healing activity. Conclusion: The methanol extract of F. amplissima bark exhibited better anti-inflammatory, wound healing and in vivo antioxidant activity most likely due to phenols constituents.