Abstract: Background: Various anabolic steroids are used to improve endurance and muscle strength. The use of such synthetic drugs is banned by World Anti-Doping Agency due to their adverse and toxic effects. On the other hand, traditional Indian medicinal plants were used by the athletes to improve their endurance and muscle strength. The objective of this study was to evaluate the effect of methanolic extract of Artemisia pallens Wall on endurance of laboratory animals. Materials and methods: Endurance in male Wistar rats (150-180 g) was achieved by treadmill running exercise. The animals were trained for two weeks. The Methanolic Extract of Artemisia pallens Wall (MEAP) was administered orally to the animals for the next 6 weeks and parameters such as CKMB, BUN, serum creatinine, serum glucose, testosterone, total protein, complete lipid profile, heart weight to body weight ratio, electrocardiographic and hemodynamic were evaluated. Lung histopathological assessments were performed. Results: Eight weeks administration of MEAP (100 and 200 mg kg-1) significantly (p<0.05) lowered the serum cholesterol and HDL levels, whereas the serum LDL and triglyceride levels were increased significantly (p<0.05). There was significant (p<0.05) decrease in serum CKMB and creatinine by MEAP (100 and 200 mg kg-1) treatment. MEAP (100 and 200 mg kg-1) was significantly (p<0.05) inhibited breakdown of protein to urea. The electrocardiographic changes like prolongation in QT and QTc intervals were seen with the MEAP (100, 200 and 400 mg kg-1) treatment. Histological aberrations were also ameliorated by MEAP treatment. Conclusion: MEAP (200 mg kg-1) significantly improved the endurance and performance of the animals.