Abstract: Background: The present investigation has been undertaken to study the in vitro and in vivo antioxidant potential of Rubus ellipticus and Rubus niveus. Materials and Methods: Leaf, stem and root of the Rubus plants was extracted in Soxhlet using petroleum ether, chloroform, acetone and methanol and all the extracts were subjected to in vitro antioxidant assays. R. ellipticus Leaf Methanol (RELM) and R. niveus Root Acetone (RNRA) extracts were administered to test the in vivo antioxidant levels after 30 days of treatment. Results: RELM and RNRA extracts depicted maximum activity in most of the radical scavenging assays such as DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2- azinobis -6-thiosulfonic acid), FRAP (ferric reducing antioxidant power assay), nitric oxide, super oxide, hydroxyl radical etc. Therefore, RELM and RNRA were taken for in vivo antioxidant screening. The 250 and 100 mg kg-1 of RELM and RNRA showed significant activities for catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione reductase and reduced glutathione. RNRA extracts showed superior antioxidant activity compared to RELM. Conclusion: This study could suggest the use of R. ellipticus and R. niveus as a valuable natural antioxidant and has immense scope as an effective source to fight against the free radical generated diseases. This study should also encourage the scientific search for more medicinal properties from these species so as to combat the ever increasing demand of medicine for the mankind.