Abstract: Backgournd: To evaluate the ameliorative effect of ethanolic seed extract of Mucuna pruriens in alloxan-induced biochemical alteration in male Wistar rats. Materials and Methods: Ethanolic seed extract of Mucuna pruriens was administered orally in doses of 5, 10, 20, 30, 40, 50 and 100 mg kg-1 body weight (b.wt.) to alloxan-induced rats. The plasma biochemical and lipid profiles and the histopathological evaluation of the liver of the normal and diabetic rats were carried out and the results compared with those diabetic rats administered with M. pruriens extract and glibenclamide (5 mg kg-1 body weight). Results: The alloxan treated rats showed significantly (p<0.05) elevated levels of plasma cholesterol, triglycerides, urea, creatinine, serum aspartate aminotransferase and serum alanine aminotransferase with concomitant decreased in total protein level when compared to the control rats. Administration of M. pruriens seed extract and glibenclamide significantly (p<0.05) decreased the plasma cholesterol, triglycerides, low density lipoprotein-cholesterol, creatinine, alkaline phosphatase levels while high density lipoprotein-cholesterol levels significantly (p<0.05) increased after 12 weeks of treatment of the diabetic rats. The effect of M. pruriens seed extract was dose dependent and the plasma and lipid profiles and the electrolytes levels were restored to near normal levels. There were no significant (p>0.05) difference in the ameliorative effect of M. pruriens extract and glibenclamide. Conclusion: Mucuna pruriens ethanolic seed extract has both hepatoprotective and lypogenic activities in alloxan induced diabetic rats and could be substituted for standard oral hypoglycaemic agent. Mucuna pruriens extract in addition to possessing hypoglycaemic properties could be used to ameliorate the biochemical alterations induced by diabetes.