Abstract: Objective: The present investigation was carried out to evaluate the safety of an hydroalcholic extract of Ipomoea aquatica Forsk (HAEIA) leaves by determining its potential toxicity after acute and sub-acute administration in rodent. Materials and Methods: For the acute study HAEIA was administered in single doses of 500, 1000 and 2000 mg kg-1 (p.o). General behavior, adverse effects and mortality were determined for up to 14 days. The sub-acute toxicity test was based on the daily administration of two doses of HAEIA (200 and 400 mg kg-1 body weight) for four weeks, 0.1% CMC served as control group. Results: In the acute and sub-acute study hydroalcoholic (Water plus ethanol) extract of Ipomoea aquatica leaves doesn’t caused any change in general behavior, adverse effects and mortality. Throughout the study period no sign of toxicity was registered. Conversely, the sub-acute doses stimulated slight increase in body weight of the animals treated test drug. Further it was observed that HAEIA at the dose of 200 and 400 mg kg-1 did not modify the weight index. It was evident that daily oral dose of HAEIA at at both the dose level for 28 day days did not shown any significant change in hematological parameters and also no change observed in biological analysis of serum Aspartate transaminase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP), bilirubin, creatinine, Blood Urea Nitrogen (BUN) levels. Conclusion: In view of the results obtained through acute toxicity and sub-acute study of Ipomoea aquatica (Water Spinach) which is consumed as a traditional medicine of india and china. No significant changes were observed in organ weights and histopathological results showed normal profile suggesting no morphological alterations there is a wide margin of safety for the therapeutic use of this plant was proved through this study which enables the research to carry out the lead invention from the natural resources.