Abstract: Objective: The study was designed to investigate the antioxidant activity along with lipid peroxidation inhibition potential of leaves of Pterospermum acerifolium (L.) Willd. Materials and Methods: The antioxidant activity of petroleum ether (PAEE) and chloroform (PACE) extract of leaves was studied by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, hydroxyl radical scavenging activity (site specific and non site specific) and Total Antioxidant Capacity (TAO%). Lipid Peroxidation Inhibition (LPO) potential of the extracts was also studied in the rat liver homogenate. Results: Results showed, 1000 μg mL-1 of PAEE showed moderate free radical scavenging activity in all the models. Subsequently, 1000 μg mL-1 of PACE showed moderate activity. In LPO assay, 1000 μg mL-1 of PAEE showed moderate inhibition percentage. Conclusion: Hence, it was evident from the results that, PAEE and PACE both at concentration of 1000 μg mL-1 showed moderate free radical scavenging activity.