Abstract: In this study, it was assessed the in vitro inhibition of sugar consumption of human saliva microorganisms by propolis extracts. Saliva samples were collected from volunteers individuals after three mouth washes with distilled water. After an oral rinsed with propolis extract I, II and III, saliva samples were collected at one, two and three h after rinsing and glucose was added to samples. These aliquots were employed to determine glucose consumption at 0, 24 and 48 h after collection. Glucose levels were determined by the glucose oxidase method. It was verified statistically significant differences in glucose consumption when comparing patients treated with propolis to GC group, after 24 and 48 h. GExpI presented glucose consumption inhibition at intervals from 0 to 24 h and 0 to 48 h at zero, one and two h after rinsing. Absence of glucose consumption was verified in GExpII and GExpIII at 0 to 24 h and from 0 to 48 h in samples collected right after rinsing, while it was observed glucose consumption at one, two and three h after rinsing. Propolis extract inhibited of sugar consumption in saliva by microorganisms and can be faced as a suitable compound to be employed in dentistry.