Abstract: Background: Inflammation and pain were associated with most of the life threatening diseases. Treatment for these conditions by available drugs producing promising therapeutic effect with more unwanted effects. To avoid this problem search of newer and alternative therapy is getting important. Sesame oil is one of the best nutrients with more therapeutic option. The context and purpose of the study: The present research was undertaken to find out the analgesic, antipyretic and anti-inflammatory activity of sesame oil (5, 10 mL kg-1 b.wt.) in experimental animals. Results: Antipyretic activity was determined by yeast induced pyrexia in rats. Sesame oil produced significant antipyretic (p<0.001) when compared with paracetamol. Analgesic and anti-inflammatory activity in few experimental models tested. Incomparable analgesic activity was found in the hot plate, acetic acid induced writhing and formalin induced pain model and it shows dose dependent activity also. In acetic acid induced writhing test the maximum percentage inhibition activity (45%) was produced by 10 mL treated mice. Conclusion: These initial findings confirm its therapeutic value against various pain and inflammatory diseases.