Abstract: This study aimed to investigate the prevalence of multidrug resistant Salmonella enteritidis from poultry chicken in comparison with the backyard country chicken along with their seasonal variation in Tiruchirappalli, Tamilnadu, India. A total of 325 rectal swab samples of chicken were included for this study. Among them 157 and 168 were from poultry and backyard chicken, respectively. Samples were randomly collected during each season like monsoon, post monsoon, Winter and Summer from July 2010 to June 2011. The total rate of isolation of Salmonella was found to be 26.8% (n = 87). Among them 32.5% (n = 51) were from poultry and 21.4% (n = 36) were from backyard chicken. Highest isolation rate was obtained during summer and the lowest, during winter. All the isolates were found to be highly resistant to β-lactam and macrolide antibiotics (52.9-100%) and highly sensitive to co-trimoxazole and ciprofloxacin (41.2-76.5%). More number of multi drug resistant Salmonella isolates was recovered from the poultry chicken from Tiruchirappalli, Tamilnadu. The probability for this may be the frequent exposure of the poultry chickens for various antibiotics in the chicken farms during their cultivation. β-lactam and macrolide antibiotics may be used in these farms routinely for curbing the bacterial infection among the chickens and this was evidenced in comparison with the backyard chicken samples.