Abstract: One hundred and thirty-five water samples were collected from five fresh water bodies in three locations in Benue State, Nigeria and screened for the occurrence of Bdellovibrio spp. using the double layer agar technique. E. coli, S. typhi and Shigella spp. were used as prey bacteria. All water bodies sampled had average temperatures ranges of 26-27°C. pH values ranged between 7 and 8. Total hardness values ranged between 48 and 85 mg L-1, while TDO values were between 5.0 and 15.3 mg L-1. A total of 53 Bdellovibrio PFUs occurred with the following frequencies on respective host prey; 42 (79.2%) on E. coli, 10 (18.86%) on S. typhi and 1 (1.88%) on Shigella spp. More research on Bdellovibrio occurrence in other niches in Nigeria is advocated to better understand the dynamics of these unique bacteria in a bid to harness its potential as control for Gram negative infections.