Abstract: Background and Objective: Gastric ulcer is prevalent in Sub-Saharan African countries where people use medicinal plants for their treatment without knowing the probable side effects. This study was carried out to evaluate the acute toxicity study, the anti-secretory and the anti-acid activities of a herbal formulation made with Macaranga barteri and Terminalia superba (AEMbTs) on pylorus ligation-induced gastric ulcers in rats. Materials and Methods: The acute toxicity study using a single dose of 5000 mg kg–1 b.wt., of AEMbTs was conducted on 10 female rats following the Organisation for Economic Co-operation and Development (OECD) (420) guidelines. As for the anti-ulcer effects, 54 rats were divided into nine groups of six rats each. The rats in Group I (non-ulcerative rats) received distilled while the other groups were administered distilled water, Maalox, cimetidine and five doses of AEMbTs (31.25, 62.50, 125, 250 and 500 mg kg–1 b.wt.), respectively. As 1 hr later, the pylori of the rats were ligated. The rats were sacrificed, the stomach contents of each rat were collected, the pH and the gastric acidity were determined, then the ulcerations were measured and the inhibition percentages of the ulcerations were calculated 6 hrs later. Results: The AEMbTs didn’t show any signs of toxicity. Hence, its 50% Lethal Dose (LD50) was greater than 5000 mg kg–1 b.wt. The results, also, indicated that AEMbTs significantly reduced the ulcer surface areas, volume and gastric acidity while increasing the gastric pH of the rats compared to those with no treatment. Conclusion: Therefore, AEMbTs are safe and have anti-secretory and anti-acid activities on pylorus ligation-induced gastric ulcers in rats.