Abstract: Background and Objective: The broad-spectrum antibacterial drugs are commonly used in food-producing animals. Suspected deposits of these drugs in products of these animals may have negative effects on human health. The present study aimed to determine the oxytetracycline (OTC) residues in broiler’s muscles and organs (heart, kidney and liver) samples. Materials and Methods: A total of 288 broiler birds were randomly allotted to four treatments T1 (20 mg kg–1), T2 (40 mg kg–1), T3 (60 mg kg–1) and T4 (control) in a Completely Randomized Design (CRD). The treatments consisted of 72 birds per treatment, divided into six replicates, with 12 birds in each replicate. Detection of oxytetracycline (OTC) residues in muscles and organs was performed by high-performance liquid chromatography with an ultraviolet detector. Results: The result at 7 days withdrawal period traces of oxytetracycline found on muscles and organs analysis were within the acceptable limit. Conclusion: During 7 days withdrawal period, traces of oxytetracycline in muscles and organs (heart, kidney and liver) of broiler birds sampled for HPLC analysis were within the acceptable limits and it is recommended that, a withdrawal period of at least 7 days should be adhered to (depending on the drug) before slaughtering the birds.