Abstract: Background and Objective: Methicillin-Resistance Staphylococcus aureus (MRSA) usually colonizes the skin, respiratory tract and urinary tract. MRSA is considered an emerging disease because it’s easily communicated from one person to another and its incidence is rising quickly. This study was undertaken to evaluate the carrier of methicillin resistance/sensitive Staphylococcus aureus producing penicillinase from health workers. Materials and Methods: Specimens were collected randomly from the health workers with a sterile swab stick from the nasal nares (nasal swab), skin swab and swab from the laboratory coat of workers (practitioners) in Irrua Specialist Teaching Hospital (ISTH), Irrua, Edo State, Nigeria. A total of 200 specimens (nasal swab, skin swab and laboratory coat swab) were randomly collected from apparently healthy male and female subjects. Results: This study, had 30 (15%) Staphylococcus aureus prevalence with the highest occurrence of 24 (30%) from skin swabs and 6 (7.5%) from nasal swabs. About 13 (43.3%) prevalence of MRSA was recorded with the highest occurrence seen in skin 9 (30%), 4 (13.3%) from nasal swabs while 17 (56.6%) of the Staphylococcus aureus isolates were Methicillin-Sensitive (MSSA). When the prevalence of MRSA and MSSA were analyzed, 7 (23%) were penicillinase negative (non-producing) and there was no significant difference. About 23 (77%) penicillinase-producing Staphylococcus aureus, 13 (57%) were methicillin/resistant and 10 (43%) were methicillin/sensitive Staphylococcus aureus with no significant difference between the two growth from penicillinase-producing. Conclusion: It was observed that methicillin-resistant/penicillinase-producing Staphylococcus aureus is significantly present in the skin and nasal nares of staff in ISTH and this may pose nosocomial infection prevalent in the hospital environment if not promptly detected and controlled.