Abstract: Background and Objective: Diabetes mellitus is a chronic metabolic disorder characterized by inappropriate hyperglycemia due to lack of or resistance to insulin and free radicals involved. Honey is known to have an antioxidant and antihyperglycemic effect in community. However, the mechanisms of rambutan honey action as antihyperglycemic have poorly understood. The objectives of this study were to investigated the effect of rambutan honey toward to decrease blood glucose level and to histopathology-changing aspects in the pancreas of diabetic Wistar rat. Materials and Methods: The research methods were an experimental laboratory. The samples (n = 30) were divided into six groups, the negative control which normal blood glucose (NC), Positive Control (PC), the group of rambutan honey 0.5, 1 and 2 g kg–1 b.wt. and glibenclamide 0.065 mg/200 g b.wt. Diabetes was induced in rats by an intraperitoneal injection of alloxan. Rambutan honey was orally administered to normal and diabetic rats for a period of 21 days. Blood glucose level was observed on day 0, 7th, 14th and 21st and the last day of the pancreas histopathologic examination stained with haematoxylin-eosin. Data were statistically analyzed using ANOVA and paired t-test. Results: Rambutan honey 0.5 g kg–1 b.wt. decrease blood glucose level significantly on day 21 (p = 0.047) with blood glucose level means of 195.2±19.83 mg dL–1 (PC), 127.6±25.46 mg dL–1 (0.5 g kg–1 b.wt.), 187.6±54.83 mg dL–1 (1 g kg–1 b.wt.) and 243.6±77.62 mg dL–1 (2 g kg–1 b.wt.), respectively and had no significant differences compared with glibenclamide. Conclusion: Rambutan honey exhibits antihyperglycemic activity in alloxan-induced diabetic rats. Thus our finding provides clear evidences that rambutan honey have a potential as an adjuvant along with antidiabetics agent treatment. However, such effects need to be confirmed on human in clinical condition.