Abstract: Background and Objective: Birth control pill is one of synthetic steroid hormones from EDCs that are believed to cause higher transgender case and cancer risk in the environment today. The objectives of this research were to (a) identify the concentrations of EDC steroid hormones in recycled wastewater used for drinking purposes and (b) investigate the influence of selective water quality parameters on the occurrence of EDC steroid hormones. Materials and Methods: Dispersive Liquid-Liquid Microextraction method was used to extract the steroid hormones and tested using High Performance Liquid Chromatography (HPLC). Results obtained from the samples will be analyzed using Microsoft Excel Office Professional Plus 2013 and Pearson Correlation. Results: As a result, the average concentration of EE2 identified was 181.63±25.12 ng mL–1. From the correlation statistical analysis, dissolved oxygen (r = -0.975, p = 0.0009) was the most influencing in-situ parameter on EE2 occurrence. Conclusion: In conclusion from the result obtained for the recycled wastewater is alarming and worrying as the steroid sex hormone is still presence and detected although in nanogram per milliliter in environment as it will bring and posed a life threat towards the living organism in the environment.