Abstract: Background and Objective: Breadfruit leaf (Artocarpus altilis) can be used to cure several diseases such as renal disease, high blood pressure, liver, diabetes, asthma, toothache, skin infection and cancer in Indonesian community. Liver disease including hepatitis and hepatotoxicity require proper management so they may heal faster. This study was aimed to examine the hepatoprotective effect of ethanol extract of Indonesian breadfruit leaf in carbon tetrachloride-induced liver injury Wistar rats by measuring Serum Glutamic Pyruvic Transaminase (SGPT) and malondialdehyde (MDA) levels. Materials and Methods: Wistar rats were divided into negative control group, positive control group and three treatment groups. Ethanol-based breadfruit leaf extract were formulated in doses of 125 mg kg–1 b.wt., (T1), 250 mg kg–1 b.wt., (T2) and 500 mg kg–1 b.wt., (T3) applied per oral for 7 days. Positive control and treatment groups were induced by 1 mL kg–1 b.wt., dose of carbon tetrachloride intraperitoneal on day 8. Data were statistically analyzed using one-way ANOVA and post hoc test. Results: The research revealed that all groups treated with breadfruit leaf have the lower of MDA and SGPT levels (p<0.05) when compared to the positive control. Ethanol-based breadfruit leaf extraction doses of 250 and 500 mg kg–1 b.wt., per oral given for 7 days before induced by 1 mL kg–1 b.wt., dose of carbon tetrachloride showed significance in decreased MDA levels (p = 0.017) however dose of 500 mg kg–1 b.wt., showed the most effective to decreased SGPT levels (p = 0.000). Conclusion: It is concluded that ethanol-based breadfruit leaf extract had effect on liver protection by decreased levels of MDA and SGPT.