Abstract: This study aims to explore amelioration of the renal toxicity induced by one of the commonly used antineoplastic agent, Cisplatin (CDDP). The therapeutic application of CDDP is limited by the induction of nephro-toxicity as a result of severe oxidative stress in renal tissues. The present study demonstrates that administration of astaxanthine (AST), a carotenoid, prevented the nephro-toxicity induced by CDDP by reducing oxidative stress in renal tissues. The renal toxicity was monitored by estimating levels of serum-biochemical parameters such as urea and creatinine. The oxidative stress in renal tissues were determined as depletion of antioxidant parameters and cellular DNA damage. The cellular DNA damages in kidney and tumor tissues were analysed using comet assay. Histopathological examinations were carried out to monitor the CDDP-induced alterations in the tissues of tumor and kidney. Histopathological studies revealed that administration of CDDP to tumor bearing mice caused morphological alterations in tumor and renal tissues. Also there was increase in the levels of serum- creatinine and urea, in these animals apart from cellular DNA damage and oxidative damage in the tissues. Administration of AST, following CDDP treatment, restored the serum parameters and prevented the morphological alterations in the renal tissues without protecting the tumor tissue. The comet assay results demonstrated that AST administration protected the cellular DNA from CDDP induced damages in the kidney cells without affecting the damage in tumor cells. The studies suggest the beneficial use of AST as an adjuvant to ameliorate the toxicity in Cisplatin-chemotherapy.