Abstract: Acanthus ilicifolius is a very potential plant and it has more phyto chemical compounds. In present study, anti microbial and hemolytic effects of Acanthus ilicifolius leaves extracts of chloroform and aqueous in isolated protein. The antimicrobial activity of crude extract against bacterial and fungal pathogens showed the clear inhibition zone against Vibrio cholerae and Aspergillus niger in chloroform extract and aqueous extracts showed clear inhibition zone for Pseudomonas sp. and Candida albicans. Both the extracts exhibited hemolytic activity which was estimated as 10.80 ht mg-1 for chloroform extract and 9.5 ht mg-1 for aqueous extract. The partial purification of protein is done by using DEAE cellulose. On SDS-PAGE the crude protein yielded three well defined bands at 100.5, 52 and 21.4 kDa in both the extracts.