Abstract: In the present study controlled release formulation of famotidine was investigated for its gastric and duodenal antiulcer activity in rats. Ulcers were produced in rats by pyloric ligation method and aspirin induced ulcer in rats. The animals were divided separately for both experiments. In each method animals were divided into four groups of six animals each. Group 1 served as normal control that received only distilled water. Group II served as disease control in which the animals were maintained under same environmental conditions but surgical manipulations done like other groups. Group III received standard drug famotidine 12 mg kg-1 orally. Group IV received famotidine formulation respectively with a dose equivalent to Famotidine 12 mg kg-1 orally as suspension. The antiulcer activity of pyloric ligated and aspirin induced animals were correlated for the reduction in ulcer levels. Parameters like volume of gastric secretion, pH, total acid and ulcer index were calculated and was concluded that the group received famotidine formulation exhibited significant antiulcer activity by both methods as compared to standard drug famotidine. The stomach biopsy of all the groups were analyzed and was found that rats which received famotidine formulation and standard famotidine showed good healing of ulcers when compared to disease control group of animals. The mean volume of gastric secretions, mean pH mean total acid and ulcer index for famotidine formulation treated group was calculated as 2.76 mL, 5.65, 112 mEq L-1 and 1.68, respectively. From the results it can be concluded that famotidine formulation exhibited significant antiulcer effect and the histopathology report also supports and confirm its effect.