Abstract: The main objective of this study is to isolate the bacterial strains which are producing biomedicinally relevant secondary metabolites. To achieve this, the ethyl acetate extracts of four marine bacterial strains BR1, PC4, EM13 and EM14 which were isolated from Balanus amphitrite (barnacle), Polyclinum constellatum (ascidian) and Enteromorpha compressa (Seaweed), respectively subjected to study the anti inflammatory, analgesic and central nervous system depressant activities. Anti inflammatory activity was studied by carragennan induced rat paw edema model. Though the results were significant (p<0.05) for all the four bacterial extracts the more effective anti-inflammatory activity was exhibited by EM13 and EM14 (range between 20-59% of inhibition). Interestingly EM13 inhibited early phases, whereas EM14 inhibited the later phases of inflammation. These two extracts produced the same effect on analgesic activity which was studied by using hotplate test. However, the ethyl acetate extracts of EM13 and BR1 showed remarkable reduction in locomotor activity and prolongation of phenobarbitone sodium induced sleeping time that demonstrated the significant CNS depressant activity. The experimental data identified that the strains EM13, EM14 and BR1 contain potential pharmacologically active compounds and suggested that to further isolation and characterization of active principles and phylogenetic identification of the epibiotic bacterial strains. The present study evidenced that the bacteria associated with marine organisms are the potential sources of pharmacologically active natural products.