Abstract: Mid Gut Gland (MGG) and Salivary Gland (SG) of B. spirata were collected separately and extracts were prepared in three volumes of 1% Acetic Acid in Methanol, centrifuged and lyophilised. The lyophilised powder was dissolved with 0.9% saline solution in a required amount and used for further assays. The assays were done in perfused isolated frog heart, isolated frog rectus abdominis muscle, frog sciatic nerve-muscle preparation, lumbar plexus of frog and in albino mice. Both MGG and SG extracts showed dose dependent effect and cholinergic principle in the perfused frog heart preparation. They didn’t alter the acetylcholine-produced contraction in the rectus abdominis muscle and they affected the sciatic nerve much than the muscle in nerve-muscle preparation which showed that they contain neurotoxins like TTX. The MGG extract proves that it has the local anaesthetic effect in the lumbar plexus experiment.