Abstract: Background and Objective: Resistance to diseases is an important step for the establishment of a genetic transformation system in plants. In current research, callus induction and establishment of cell suspension culture were investigated in Cuminum cyminum. Materials and Methods: Callus inducted from hypocotyls segments. The experiment was arranged in a factorial experiment with 2,4-Dichlorophenoxyacetic acid (2,4-D) and Kinetin (KIN) different concentrations (0, 0.1, 1 and 10 μM) on MS medium. Callus was inducted 95.6% on MS medium supplements with 2, 4-D 0.1 and KIN 10 μM L–1. The cell suspension culture was established from the callus of Cumin GC-4. Using different concentrations and combinations of 2,4-D and kinetin the growth patterns of cell suspension cultures were examined during a range of culture periods (0, 7, 14, 21 and 28 days). Results: The growth rates of cells were initially slow, but as the culture proceeded, they increased significantly and accumulated great amounts of biomass over a period of 28 days. Conclusion: Friable callus production is important in establishment of good quality of cell suspension. Researcher can take benefit from its investigation to manipulation genetically.