Abstract: Background and Objectives: Soybean (Glycine max. (L.) Merr) is a highly valued source of protein and vegetable oil, grown extensively in India as well as globally and gaining importance as a nutraceutical. The objective was to study the influence of vesicular arbuscular mycorrhiza (VAM) on growth, phenolic compound, isoflavone folic acid content and antioxidant activities to enhance nutraceutical value of soybean. Materials and Methods: Soybean seeds, variety JS-335 were germinated in association with VAM fungi Glomus fasciculatum (G. fasciculatum) and Glomus mosseae (G. mosseae) grown in pots containing sand-soil mixture (1:1) with respective VAM inoculants and controls. Plants were grown for 9 weeks with weekly observations and data were recorded with respective height of each plant, corresponding dry weight, pods weight per plants. The control and treated plants were analyzed for phenolic compound, flavonoids and antioxidant activities. And isoflavone content was quantified by using HPLC method. Results: Treatment of VAM resulted in increase in fresh weight (approx. 2.5 fold) and dry weight of 0.8-2.2 folds. Similar increase in seed weight per plant was obtained under VAM treatment. Flavonoid levels, folic acid, isoflavone content and phenolic contents were higher in the VAM treatments in comparison to control. Soybean extract showed great ability to scavenge free radicals and antioxidant activities. Conclusion: Seeds of soybean showed the presence of bioactive compound such as flavonoid, folic acid, phenolic, isoflavone and antioxidants. Profiling of phytochemicals in soybean seeds in response to VAM treatment will help to select suitable cultivars for their utilization in the preparation of value added processed soy products, thereby enhancing the health benefits of soy and soy-based foods for human consumption.