Abstract: Background and Objective: In agricultural eco-systems, microorganisms play a vital role in nutrient recycling through mobilization and solubilisation of mineral nutrient and biological nitrogen fixation. Symbiotic relation of soil microbes and legumes offer an alternative source to chemical fertilizer for plant growth and yield. The aim of the study was to assess the ability of soybean genotypes to nodulate with indigenous rhizobial inoculants. Materials and Methods: Field experiment was performed in four soybean cultivars with different rhizobial combinations in 2 experiments. In first experiment, soybean cultivar JS335 was inoculated with six rhizobia and two doses (30 and 60 kg) of N-fertilizer separately, while in second experiment evaluation of five isolates with three soybean cultivars were done in order to observe the effect of different inoculation and cultivar combination. The Data were analysed with one- and two-way analysis of variance (ANOVA) separately for first and second experiment, respectively. Results: The results showed significant difference (p<0.001) in symbiotic performance among the inoculants associated with soybean cultivars JS335. All test isolates were more effective and enhance 18.22-29.21% grain yield as compared to uninoculated control. Except isolate ALSR3, all test isolates enhanced 4.6-14.4% grain yield as compared to 60 kg ha–1 N-fertilizer. Soybean cultivar and inoculation combinations showed effective nodulation which were significantly different (p<0.001) from the uninoculated plots. The symbiotic parameters were much greater with cultivars VL47 and DS9712 in terms of nodule number, nodule dry weight and pod numbers plant. But non-significant difference was observed in grain yield. Conclusion: It was concluded that taken together, the symbiotic performance and grain yield data showed cultivar and inoculant specific effects and suggest that selection of rhizobial strains must be appropriate for the cultivars and newly released varieties.