Abstract: The present study was conducted to examine the effect of pretreatment with cycloheximide on isolated flowers as well as on cut scapes of Hemerocallis fulva followed by their transfer to different holding solutions. The scapes were harvested, brought to laboratory and pulsed for 1 h with different concentrations of cycloheximide (CHI). Following the treatment, they were transferred to Distilled Water (DW) or sucrose+cobalt chloride (DW or SUC+CoCl2). In case of isolated flowers, cycloheximide was sprayed at different concentrations immediately after flower opening, under laboratory conditions. Pulsing the cut spikes with CHI at 0.01 or 0.05 mM CHI before transfer to Suc+CoCl2 enhanced their longevity, promoted the opening of buds in a sustained manner and maintained higher fresh and dry mass of flowers besides maintaining the tissue content of soluble proteins. Pretreatment with CHI did not increase the longevity of isolated flowers instead the increase in vase life was due to the profusion and continuity with which buds bloom into flowers. However, spraying isolated flowers with 0.5 mM CHI increased their longevity upto 3 days as compared to 1 day in unsprayed flowers. In the present study, increased vase life was found associated with decrease in the tissue content of total phenols. Pretreatment of scapes (0.01 and 0.05 mM) as well as spraying isolated flowers with CHI (0.5 mM) before transfer to holding solutions resulted in an enhancement of vase life/flower longevity, maintenance of membrane integrity and improving postharvest performance of cut scapes and isolated flowers of Hemerocallis fulva.