Abstract: Using the method of liquid culture and selecting the variety of Handao502, the effect of Steviol glycosides Blending Liquid (SBL) on seeding growth and development in upland rice had been studied in phytotron. Many indexes were studied including the germination rate of seed, the fresh weight and dry weight, the indexes of physiology and biochemistry in seeding stage. The results indicate that the appropriate treatment of SBL increases the seed germination rate, promotes the growth of shoot and the roots in the seeding stage, increases the fresh weight, dry weight and the radio of root to shoot, increases the peroxidase (POD) activity, the free proline content and the root vigor, decreases the malondialdehyde (MDA) content. So, proper SBL increases the unsuitable resistance ability in the growth and development of seeding stage in upland rice. This study provides a certain foundation for the high yield cultivation of upland rice.