Abstract: In the present study, the antibacterial activity of six different extracts of tuber of Zehnaria scabra was evaluated by agar diffusion method in Muller-Hinton agar medium. The crude ethanolic and methanolic extracts inhibited the growth of all the pathogenic bacteria used in the present study. Benzene and chloroform extracts of Z. scabra exhibited moderate antibacterial activity, where as petroleum ether and hexane extracts showed the least antibacterial activity. Petroleum ether and hexane extracts inhibited the growth of Bacillus subtilis and Staphylococcus aureus, respectively. The gram positive bacteria were more susceptible to all the crude extracts screened for its antibacterial activity. Pseudomonas aeruginosa, gram negative and multidrug resistant bacteria was sensitive to almost all the extracts, but E. coli was more susceptible to the crude extracts and showed higher antibacterial activity by producing the higher diameter of inhibitory zone. The inhibitory zone diameter produced by E. coli was ranged from 10 to 14 mm. The crude extracts have greater potential and can be used for controlling the growth of methicilin resistant bacteria Staphylococcus aureus and multi drug resistant nosocomial pathogen Pseudomonas aeruginosa.