Abstract: The sensitivity potential of the bacteria Salmonella typhi and Staphylococcus aureus to Eucalyptus citrodora (Lemon) and Eucalyptus camadulensis (menthol) fractions was investigated. The whole extract samples were first evaluated for anti-microbial properties using the cup plate method. The whole oil extract samples showed high antimicrobial activity on the Salmonella typhi and Staphylococcus aureus. The whole extracts were then fractionated and each fraction was collected at its boiling point range. The sensitivity potential of the test organisms was then carried out in these fractions collected. The results obtained indicated that Salmonella typhi is highly sensitive to the fraction 3 (bpt) 152-154°C of Eucalyptus camaldulensis and moderately sensitive to all the other fractions of Eucalyptus camaldulensis. However all the fractions obtained from E. citrodora except fraction (I) and the residual extract were very effective on the Salmonella typhi. Meanwhile Staphylococcus aureus showed very high sensitivity to all the fractions obtained from the two whole extracts of E. citrodora and E. camadulensis.