Abstract: This study describes the investigation on the synthetic seed technology in two varieties (Loda and China) of eggplant with a particular focus on the carbon source in seed bead. To assess exact type and concentration of carbon source, three types of carbon sources (viz., sucrose, mannitol and sorbitol) and their different concentrations were used in alginate seed bead to achieve the optimum germination of synthetic seed on MS0. Among the different carbon sources, sucrose showed better performance for both varieties when nodal segment with bud was used as explants. But when somatic embryos were used as explants sucrose + sorbitol (1:1) was found to be more efficient for both varieties in germination of synthetic seeds. On the other hand, among the different concentrations 1% sucrose gave good result for the germination of synthetic seed made of nodal segment. In case of somatic embryo 3% sucrose showed best performance for both varieties.