Abstract: Aloe vera leaf gel is widely used as a traditional folk medicine for the treatment of different infectious diseases. In vitro antimicrobial properties of ethanolic extract of Aloe vera leaf gel were investigated against various common pathogenic bacteria and fungi. The well and disc-diffusion method showed significant zone of inhibition against all the pathogens studied and the results are comparable to the conventional antibiotics. These results support the ethanomedicinal use of Aloe vera for the treatment of various ailments.