Abstract: Ten tomato (Lycopersicon esculentum) genotypes showing distinct variation in morphological and anatomical features were screened for random amplified polymorphic DNA (RAPD). Germplasm under study comprised 3 mutant derivatives, 3 hybrids, their parents and one wild cherry tomato genotype. Twelve random decamer operon primers of OPAB series could generate total 690 bands of which 33.3% were polymorphic. Mutant specific polymorphic markers were detected. Polymorphy could clearly identify mutant derivatives, cultivated genotypes and wild cherry tomato genotype. The UPGMA based dendrogram divided genotypes into two main clusters. Interestingly, wild cherry tomato was present in cluster of mutant derivatives, affirming its potential for utilization as unique source for tomato breeding. Low level of genetic diversity was noted in cultivated tomato genotypes (Mean = 0.138) indicating the existence of limited or narrow genetic base.